Training Programs

Project/Dissertation work for Lifescience students

We are providing assistance for project/dissertation work for lifescience students in post-graduate and under-graduate programmes towards the partial fulfillment of their degrees.This will give the students an opportunity for industrial exposure as well as real hands-on training in the frontier areas of biotechnology and molecular biology.

Focus Areas:
Genomics
Molecular Biology
Molecular Taxonomy
Biotechnology
Microbiology
Biochemistry

Skill Development Program

Primordia Lifesciences is conducting hands on training programme in Molecular biology techniques. This training programme is designed as a finishing school for Lifescience graduates and post graduates to enhance the employability in Biotech industries as well as for researchers and academicians who want to enhance their skills. We have training programmes in two different formats: Basic and Advanced.

Learning Outcomes
  • Get familiar with day-to-day working of a Molecular biology laboratory.
  • Gain subject specific skills through laboratory based biological tools and techniques.
  • Get trained to carry out laboratory procedures correctly and independently.

Two Weeks Training In Molecular Biology

Basic Molecular Biology Techniques
  • Calculation and preparation of buffers
  • Genomic DNA isolation from bacteria
  • Agarose gel electrophoresis of Genomic DNA
  • Total RNA extraction from bacteria
  • Agarose gel electrophoresis of RNA
  • Plasmid DNA isolation
  • Plasmid DNA gel electrophoresis
  • Polymerase Chain Reaction from bacterial DNA
  • Agarose gel electrophoresis of PCR product
DNA Barcoding
  • Genomic DNA isolation from plant tissue
  • Agarose gel electrophoresis
  • Primer Designing
  • Polymerase Chain Reaction from plant DNA
  • Agarose gel electrophoresis of PCR product
  • Gel extraction of PCR product
  • Agarose gel electrophoresis of purified PCR product
Bioinformatics and Data Analysis
  • Sequence quality check and trimming
  • Use of software/tools
  • BLAST analysis for species identification
  • Phylogenetic tree construction

Advanced Techniques

Training I

Types Of Pcr (1week)

  • PCR
  • Colony PCR
  • Gradient PCR
  • RNA Isolation and cDNA Synthesis
  • RT-PCR
  • Gel Electrophoresis
Training II

Barcoding (1 Week)

  • DNA isolation
  • Primer Selection and Designing
  • Agarose Gel Electrophoresis
  • PCR
  • PCR product purification
  • Sequence Analysis and Submission to NCBI
Training III

Molecular Cloning (2weeks)

  • Competent cell preparation
  • Preparation of DNA for Cloning
  • Quantification
  • Ligation
  • Transformation
  • Plasmid Isolation
  • Sequence analysis
Training IV

Real Time Pcr (3 Days)

Theory

  • Introduction to Real-Time PCR
  • Primer & Probe Designing

Practicals

  • Primer Designing for real time
  • RNA Isolation & cDNA Synthesis
  • Absolute Quantitation (Lab)
  • Relative Quantitation (Lab)
  • Data Analysis & Interpretation
    • Training V

    Cell Culture Hands-on Training (4 Days)

    Cell Culture Basics

    • Introduction to Cell Culture
    • Types of Cell Cultures
    • Sterile Techniques
    • Lab Equipment and Reagents

    Media Preparation and Sterile Techniques

    • Preparation of Cell Culture Media
    • Sterile Handling
    • Autoclaving and Sterilization:

    Cell Line Maintenance and Subculturing (Passaging)

    • Handling Cell Cultures
    • Cell Counting and Viability
    • Cryopreservation of Cells:

    Cell Morphology and Growth Monitoring

    • Microscopic Observation
    • Contamination Detection

    Biotech Industrial Training/internship (1 Month)

    Basic Molecular Biology Techniques DNA Barcoding Bioinformatics and Data Analysis Competent Cell Preparation and Transformation Hands on Training in Biotech -Manufacturing
    • Calculation and preparation of buffers
    • Genomic DNA isolation from bacteria
    • Agarose gel electrophoresis of Genomic DNA
    • Total RNA extraction from bacteria
    • Agarose gel electrophoresis of RNA
    • Plasmid DNA isolation
    • Plasmid DNA gel electrophoresis
    • Polymerase Chain Reaction from bacterial DNA
    • Agarose gel electrophoresis of PCR product
    • Genomic DNA isolation from plant tissue
    • Agarose gel electrophoresis
    • Primer Designing
    • Polymerase Chain Reaction from plant DNA
    • Agarose gel electrophoresis of PCR product
    • Gel extraction of PCR product
    • Agarose gel electrophoresis of purified PCR product
    • Sequence quality check and trimming
    • Use of software/tools
    • BLAST analysis for species identification
    • Phylogenetic tree construction
    • Competent cell preparation Conventional method (theory)
    • Competent cell preparation using a commercial kit.
    • Transformation of plasmid DNA into competent cells.
    • Preparation of LB agar plates supplemented with ampicillin
    • Plating transformed cells and incubation.
    • Isolation of plasmids from positive transformed colonies.
    • Agarose gel electrophoresis of isolated plasmids
    • Introduction & raw material management
    • Batch production.
    • Good Documentation Practices (GDP)
    • Quality control (QC) testing
    • Preparation of SOPs.
    • Batch Manufacturing
    • Record (BMR) completion practice.
    • Packaging, labeling, and storage under GMP conditions.

    Project/dissertation Work For Lifescience Students

    We are providing assistance for project/dissertation work for lifescience students in post-graduate and under graduate programmes towards the partial fulfillment of their degrees. This will give the students an opportunity for industrial exposure as well as real hands-on training in the frontier areas of biotechnology and molecular biology.

    Focus AreasGenomics | Molecular Biology | Molecular Taxonomy | Biotechnology | Microbiology

    Program Highlights
    • Highly experienced and qualified faculty members.
    • State-of-the-art laboratory equipped with advanced tools and techniques.
    • Air-conditioned lab environment for optimal comfort.
    • Comprehensive support provided for thesis preparation and writing.

    NOTE: For more details regarding fee structure contact us on- 8943124504